Merck SeQuant® HILIC Columns


Merck Complete Range for Separations of All Polar Hydrophilic Compounds

SeQuant® HILIC (hydrophilic interaction liquid chromatography) HPLC columns constitute a range of high-performance tools for separating polar hydrophilic compounds. All columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. Separation is achieved by hydrophilic partitioning combined with weak ionic interactions for maximum selectivity, high loadability and easy optimization of methods.

Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example has US FDA (United States Food and Drug Administration) recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.

What is HILIC?
HILIC stands for hydrophilic interaction liquid chromatography. The technique combines the benefits of aqueous mobile phases, like reversed-phase HPLC, with separation based on normal phase principles. As a result, more polar compounds respond with higher retention resulting in more efficient separation. This makes HILIC the method of choice for separating charged and neutral hydrophilic compounds, such as acids, bases, ions, peptides, metabolites and sugars, which are very difficult to separate using reversed-phase HPLC. The HILIC HPLC separation technique also offers greater flexibility when analyzing polar hydrophilic compounds, as it is easy to combine with a wide range of detection techniques. What’s more, HILIC boasts improved sensitivity with some of these techniques, such as electrospray ionization mass spectrometry (ESI-MS).

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Application Examples Using SeQuant® HILIC Columns
SeQuant® HILIC columns have been used in numerous methods for analysis of a wide variety of polar hydrophilic compounds such as acids and bases, anions and cations, carbohydrates, metabolites, metal complexes, amino acids, peptides, and protein digests. Several of these methods have been collected into application compilations available to you below.

Further Application Examples Using SeQuant® HILIC HPLC Columns.

  • Chromatography Application World
  • SeQuant® scientific papers
  • SeQuant® application notes
  • Analytical Chromatography Posters
  • US FDA analysis of melamine and related potential contaminations
  • Glycomics
  • Peptide mapping
  • Sugar analysis
  • SeQuant® HILIC Product Range

Merck Millipore offers a comprehensive range of high-quality SeQuant® HILIC HPLC columns for the separation of polar hydrophilic compounds. Every SeQuant® HILIC HPLC separation column by Merck Millipore is individually tested and delivered with a certificate documenting its performance.

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Technical Support for SeQuant® HILIC Columns
Merck Millipore offers several technical support services to help you achieve optimal separations and reliable analytical results with SeQuant® HILIC products.

SeQuant® FAQ list
Detailed instructions

SeQuant® column Functional group Features Base particle Particle size Pore size Column types
High-performance selectivity and robustness silica 3.5 µm,
5 µm
100 Å, 200 Å analytical,
Complementary selectivity with favorable LC-MS performance silica 3 µm 100 Å analytical, capillary,
High pH-stability and low noise with ion
polymer 5 µm analytical,